The existing researches focused on using the commercial full wavelength spectrometer to determine the quality parameters of meat by detecting the meat emulsion, which were difficult to achieve online and non-destructive detection of the moisture content of intact meat. Moreover, the accuracy of pieces moisture detection is low, and people did not consider differences in the organizational structure of the pork meat itself. In this paper, we have developed a portable data acquisition system based on discrete wavelengths of spectral, and used it to detect the moisture content of fresh intact pork meat within a certain depth range. Based on the steady-state spatially resolved spectroscopy and considering the muscle fiber structure and direction of intact pork meat, we have designed a device with a symmetrical structure, which has a wavelength of 1300nm, 1450nm, 1550nm and 970nm LED light source for detecting the moisture content of the samples obtained from theLongissimus, within a certain depth range, and verified the stability and linearity of the system. The results show that the coefficient of determination is 0.49, and the detection range is 73.19%~77.654%. This study shows that scattering properties of meat is one of the main factors affecting the stability of detection.